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Mishra Mithilesh, N.
- Differentiation of Bone and Soft Tissue Using Methylene Blue - Acid Fuchsin: a New Stain Combination
Authors
1 Department of Oral Pathology, KLE VK Institute of Dental Sciences, KLE University, Nehru Nagar, Belgaum-590010, Karnataka, IN
2 Department of Oral Pathology, Bharatiya Vidhyapeeth Dental College, Sangli, Maharashtra, IN
3 Dept. of Pathology, JN Medical College, Belgaum-10, IN
4 Department of Oral Pathology, ITS Dental College & Hospital, Noida, IN
Source
Indian Journal of Public Health Research & Development, Vol 3, No 1 (2012), Pagination: 182-184Abstract
Introduction
Hematoxylin-Eosin(H-E) provides optimal staining for histology, but in formalin-fixed, decalcified, paraffinembedded bone tissue, both bone and soft tissue appear the same shade of pink. In addition to the improved staining properties, the methylene blue-acid fuchsin stain is also superior to H-E in terms of its stability, cost and ease of use.
Aim & Objectives
To determine the efficacy of methylene blue-acid fuchsin stain combination to differentiate mineralized structures from surrounding soft tissues in various oral lesions.
Material & Methods
Two sections each of peripheral cemento-ossifying fibroma, central cemento-ossifying fibroma, fibroma with calcification, fibrous dysplasia, adenomatoid odontogenic tumour and pleomorphic adenoma were taken. One section was stained with hematoxylin-eosin and the other with methylene blue-acid fuchsin.
Results & Conclusion
In contrast to standard hematoxylin-eosin stain, which stains both bone and soft tissues pink, the methylene blue-acid fuchsin stain combination demonstrates remarkable contrast with bone appearing bright pink and the soft tissue blue-purple.
Keywords
Methylene Blue, Acid Fuchsin, Bone, Soft TissueReferences
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- Comparison and Correlation of Glucose Levels in Serum and Saliva of Patients with Diabetes Mellitus
Authors
1 Department of Oral Pathology, KLE VK Institute of Dental Sciences, KLE University, Belgaum, IN
2 Department of Oral Pathology, Goa Dental College & Hospital, Goa, IN
3 Department of Pathology, JN Medical College, KLE University, Belgaum, IN
4 Department of Oral Pathology, ITS Dental College & Hospital, Noida, IN
Source
Indian Journal of Public Health Research & Development, Vol 2, No 1 (2011), Pagination: 103-105Abstract
Background and objectives
In Diabetes Mellitus, an important aspect of glycaemic control is to regularly monitor glucose levels. Current methods employed, either require a blood sample or urine sample. These procedures usually cause pain and discomfort to the patient. Hence, arises the need for a noninvasive technique in diagnosis and in monitoring glycaemic status of an individual. The study was undertaken in an attempt to compare and correlate glucose levels in saliva and serum of patients with diabetes and non-diabetic healthy individuals, to determine the efficacy of saliva as a diagnostic aid.
Method
250 individuals visiting diabetic clinics were screened randomly. Of these, 200 were confirmed diabetics and were under medication (Study Group). The remaining 50 gave neither a past history of diabetes nor did their present glycemic status depicted high values (Control Group). Venous blood and salivary samples were obtained from each individual and subjected to glucose estimation. Both fasting and post-prandial samples were analyzed.
Results and observations
Glucose was detected in the saliva of both diabetic and non-diabetics. The fasting salivary glucose values in the control group ranged from 4.1 to 13.3 mg/dl and the postprandial salivary glucose values from 12.5 to 20.0 mg/dl. The fasting salivary glucose values in the study group ranged from 4.1 to 26.6 mg/dl and the Post-prandial salivary glucose values from 15.3 to 30.7 mg/dl. It was observed that as blood glucose levels changed in both fasting and post-prandial samples, so did salivary glucose levels, irrespective of age and sex. A significant P value < 0.001 and positive correlation was found between blood glucose and salivary glucose levels in both the diabetics and the controls.
Conclusion
It can thus be inferred that saliva can be used as an adjunct diagnostic tool in Diabetes Mellitus.
Keywords
Diabetes Mellitus, Glycaemic Status, Salivary Glucose, Non Invasive Diagnostic Tools in Diabetes, Saliva in DiabeticsReferences
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